Description
The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.
Standard kits include KAPA2G Robust (5 U/µL), KAPA2G Buffer A, KAPA2G Buffer B and KAPA2G GC Buffer (all at 5X, with 1.5 mM MgCl2 at 1X), KAPA Enhancer 1 (5X), and supplemental MgCl2 (25 mM). Kit options include HotStart and dNTP Mix (10 mM each).
ReadyMix formulations (2X) of KAPA2G Robust HotStart DNA Polymerase are also available. These contain DNA Polymerase, reaction buffer (with or without dye), dNTPs and MgCl2 (at a final concentration of 2 mM).